![SOLVED: QNo.3. Calculate Tm of the following primers: Gene: Sequence Melting temperature Forward primer: TCGAGTCGCGTCCACC Reverse primer: GGGAGCATCGTCGCCC Amplified product length = b. Screen shot of in-silico PCR = Cytogenetic location = SOLVED: QNo.3. Calculate Tm of the following primers: Gene: Sequence Melting temperature Forward primer: TCGAGTCGCGTCCACC Reverse primer: GGGAGCATCGTCGCCC Amplified product length = b. Screen shot of in-silico PCR = Cytogenetic location =](https://cdn.numerade.com/ask_images/479db41e130a4d759330f82f59f9f3f4.jpg)
SOLVED: QNo.3. Calculate Tm of the following primers: Gene: Sequence Melting temperature Forward primer: TCGAGTCGCGTCCACC Reverse primer: GGGAGCATCGTCGCCC Amplified product length = b. Screen shot of in-silico PCR = Cytogenetic location =
![Nucleic Acid Design Applications Polymerase Chain Reaction (PCR) Calculating Melting Temperature (Tm) PCR Primers Design. - ppt download Nucleic Acid Design Applications Polymerase Chain Reaction (PCR) Calculating Melting Temperature (Tm) PCR Primers Design. - ppt download](https://images.slideplayer.com/18/5707607/slides/slide_13.jpg)
Nucleic Acid Design Applications Polymerase Chain Reaction (PCR) Calculating Melting Temperature (Tm) PCR Primers Design. - ppt download
![SOLVED: Use the Wallace Rule equation to calculate the Tm of each of the primers you designed. Tm = 2(A+T) + 4(G+C) Left primer: 5' CCTGGACACTGTAC 3' Right primer: 5' AATGTATGAACCTG 3 SOLVED: Use the Wallace Rule equation to calculate the Tm of each of the primers you designed. Tm = 2(A+T) + 4(G+C) Left primer: 5' CCTGGACACTGTAC 3' Right primer: 5' AATGTATGAACCTG 3](https://cdn.numerade.com/ask_previews/36c6a3fa-5970-4100-bf21-f3effc639f21_large.jpg)